In the first two issues of popular science, the mainstream chemiluminescence technology in immunodiagnosis was introduced, and it was also understood that chemiluminescence is divided into homogeneous chemiluminescence and heterogeneous chemiluminescence according to whether separation and cleaning steps are required. Compared with the traditional heterogeneous immunoassay mode, homogeneous chemiluminescence can further improve the efficiency of clinical detection and simplify the operation steps.
Today we focus on the 5th-generation homogeneous chemiluminescence platform developed by Poclight Biotechnology based on CRET technology.
Chemiluminescence - intensification of involution
In the field of chemiluminescence, the phenomenon of involution is intensifying, and the industry is facing a dilemma. With the continuous development of technology and the intensification of market competition, enterprises and research institutions are facing tremendous pressure and challenges. The causes of chemiluminescence involution are analyzed as follows:
(1) The market side has continuously increased requirements for the degree of equipment automation, and the product price is required to continue to decline, resulting in an increase in enterprise costs, insufficient ability to compress production costs, and a decrease in capital sources, which is a dilemma;
(2) You can't reduce costs quickly, and you can only choose to continue to roll in or gradually go out;
(3) Although the multi-channel single-person luminometer solves the problem of instrument cost, the reagent needs to be refrigerated and stored, and the transportation cost will be greater than the cost of the reagent;
(4) Chemiluminescence has certain requirements for detection operations, and the level of operators in the sinking market is uneven, and sometimes the detection results cannot be accurately analyzed and controlled, and the equipment has a complicated liquid path, resulting in greater after-sales pressure.
Break the involution - only innovation
It has been said that "it is never a better carriage that beats a horse-drawn carriage, but a newer application of technology, such as a steam car". It can be seen that technological innovation is the core competitiveness. The development of immunoassays is a story of innovation and development. Since 1960, the development of immunodiagnostic technology has been continuously updated and iterative, starting from the earliest radioimmunology, it has undergone colloidal gold, enzyme-linked immunoassay, chemiluminescence and other technologies to meet the needs of clinical diagnosis.
Poclight CRET homogeneous chemiluminescence – breaks out of the roll
System composition
Two antibody-DNA complexes (antibody 1-DNA1, antibody 2-DNA2), acridine ester (AE)-labeled DNA3 (DNA1, DNA2, and DNA3 single strands each contain 7–9 bases each paired in pairwise pairing), endonuclease, and graphene oxide (GO)
Brief description of the principle
(1) When the sample contains the target antigen, the antibody (Ab) in the two antibody-DNA complexes will recognize the analyte at the same time, and the sandwich structure of the antibody-antigen-antibody makes DNA1 and DNA2 close enough to hybridize the paired bases of DNA1 and 2, and then both DNA1 and DNA2 can hybridize with DNA3 to form an ortho complex.
(2) Once DNA3 forms a double-stranded structure, GO cannot adsorb DNA3, and when hydrogen peroxide is added, the chemiluminescence of AE on the ortho complex cannot be quenched by graphene oxide.
(3) The endonuclease introduced at the same time makes the chemiluminescence signal continuously cyclically amplified.
(4) In the absence of analyte, DNA3 is absorbed on the surface of GO by vulture-vulture stacking, and when hydrogen peroxide is added, the chemiluminescence of acridine ester attached to the end of single-stranded DNA3 is quenched by graphene oxide without affecting the results.
The 5th-generation chemiluminescence platform has been developed by poclight based on CRET technology, and the CRET detection system maximizes the use of graphene oxide characteristics, eliminates the magnetic beads used in the traditional chemiluminescence detection process, eliminates the separation and cleaning steps, and does not have complex liquid paths, making the instrument structure more concise. The fusion of nucleic acid technology in immunodiagnosis, so that the nucleic acid proximity touch effect plays a role, through the enzyme digestion cycle amplification technology, the detection sensitivity is improved by 1-2 orders of magnitude, and the performance is further improved.
Poclight, as a manufacturer, it has independent research and development patents. And our products, including POClia Automatic Chemiluminescent Immunoassay, and Rapid Clinical Immunoassay POCT Analyzer for Hospital and lab, as a POCT 5-10 Minutes Benchtop Immunoassay Analyzer supplier, can bring you fast, accurate and easy tests. If you are interested in our China OEM Customized Laboratory Clinical Emergency Portable Poct Micro Chemiluminescence Immunoassay Analyzer, welcome to contact us, and we can provide you with trusted supplier of lab and poct solutions.
